0050652
print

Extractable Nuclear Antigen Antibodies (Smith/RNP, Smith, SSA 52, SSA 60, and SSB)

ENA ABS4
Example Reports
Negative
Positive

Ordering Recommendation

Recommended for the differential diagnosis of systemic lupus erythematosus (SLE) and Sjogren syndrome.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation
Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.3 mL)

Storage/Transport Temperature

Refrigerated..

Unacceptable Conditions

Plasma or other body fluids. Bacterially contaminated or severely lipemic specimens.

Remarks
Stability

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 month (avoid repeated freeze/thaw cycles)

Methodology

Quantitative Enzyme-Linked Immunosorbent Assay/Quantitative Multiplex Bead Assay

Performed

Sun-Sat

Reported

1-3 days

Reference Interval

Test Number
 Components
 Reference Interval
  Smith/RNP (ENA) Ab, IgG 19 Units or less
  Smith (ENA) Antibody, IgG 40 AU/mL or less
  SSA-52 (Ro52) (ENA) Antibody, IgG 40 AU/mL or less
  SSA-60 (Ro60) (ENA) Antibody, IgG 40 AU/mL or less
  SSB (La) (ENA) Antibody, IgG 40 AU/mL or less

Interpretive Data




Component Interpretation
Smith/RNP (ENA) Antibody, IgG 19 Units or less            Negative
20-39 Units                   Weak Positive
40-80 Units                   Moderate Positive
81 Units or greater      Strong Positive
Smith (ENA) Antibody, IgG 29 AU/mL or less          Negative
30-40 AU/mL                 Equivocal
41 AU/mL or greater    Positive
SSA-52 (Ro52) (ENA) Antibody, IgG 29 AU/mL or Less          Negative
30-40 AU/mL                   Equivocal
41 AU/mL or greater      Positive
SSA-60 (Ro60) (ENA) Antibody, IgG 29 AU/mL or Less          Negative
30-40 AU/mL                   Equivocal
41 AU/mL or greater      Positive
SSB (La) (ENA) Antibody, IgG 29 AU/mL or less            Negative
30-40 AU/mL                   Equivocal
41 AU/mL or greater      Positive

Compliance Category

FDA

Note

Hotline History

View Hotline History

 

Hotline History

Date of Change
Test Name Change
Methodology
Performance/Reported Schedule
Specimen Requirements
Reference Interval
Interpretive Data
Note
CPT Code
Component Change
Other Interface Change
New Test
Inactive
02/21/2023
X
N/A

CPT Codes

86235 x5

Are you an ARUP Client? Click here for your pricing.

Components

Component Test Code* Component Chart Name LOINC
0050085 Smith (ENA) Antibody, IgG 18323-6
0050470 Smith/RNP (ENA) Ab, IgG 33983-8
0050691 SSA-52 (Ro52) (ENA) Antibody, IgG 31625-7
0050692 SSB (La) (ENA) Antibody, IgG 29965-1
2012055 SSA-60 (Ro60) (ENA) Antibody, IgG 53019-6
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • Antibodies to Extractable Nuclear Antigens
  • Ro Ab (ENA)
  • Ro52
  • Ro60 (ENA)
  • Sm Smith Autoantibodies
  • Sm/RNP (ENA)
  • Sm/RNP (ENA) Antibody, IgG
  • Smith (ENA) Antibody, IgG
  • SmRNP Autoantibodies
  • SSA (Ro) (ENA) Antibody, IgG
  • SSA Ro
  • SSA-52
  • SSA-60
  • SSB (La) (ENA) Antibody, IgG
  • Topo-1
  • topoisomerase 1
Extractable Nuclear Antigen Antibodies (Smith/RNP, Smith, SSA 52, SSA 60, and SSB)